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Objective To compare the diagnostic accuracy of magnifying chromoendoscopy (MCE) and endoscopic ultrasonography (EUS) for preoperative endoscopic assessment of the invasion depth of colorectal laterally spreading tumour(LST).Methods Data of 104 cases of colorectal LST were included.With the final pathological diagnosis as the golden standard,the accuracies of MCE and EUS for preoperative assessment of the invasion depth of colorectal LST were compared.Results The diagnostic accuracies of MCE and EUS for evaluating the invasion depth of LST were 89.4％ (93/104) and 73.1％ (76/104),respectively(P＜0.05).The lesion size and the endoscopist could affect the accuracy of the EUS evaluation (P=0.017,OR=3.561;P=0.035,OR =1.399).The accuracy of EUS seemed to show a downward trend for colorectal LST of larger diameters.Conclusion Both MCE and EUS are effective for evaluating the invasion depth of colorectal LST,but the accuracy of MCE may be higher than that of EUS.Large diameter of the lesion and the doctor's experience inadequacy may be the risk factors for the accuracy of EUS.
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Objective To observe the expression of ZNF139 and MMP-7 in endometrial adenocarcinoma tissues and discuss the correlation with clinical pathological significances.Methods In 71 patients diagnosed as endometrial adenocarcinoma,71 cancer tissues(adenocarcinoma group) and 71 adjacent normal tiuues(adjacent cancer group) were collected in operations.Immunohistochemical methods was used to detect the expression of ZNF139 and MMP-7 in the two groups,the relationship between the two genes and communication with clinicopathological indexes were discussed.Western blot and qRT-PCR were used to detected the expression of ZNF139 and MMP-7 at protein and mRNA level.Results The positive rates of ZNF139 was 66.20% in adenocarcinoma group,higher than in adjacent cancer group(21.42%,P=0.016);the positive rates of MMP-7 was 74.65% in adenocarcinoma group,higher than7 than in adjacent cancer group(26.76%,P=0.012).The expression of ZNF139 and MMP-7 was correlated with lymph node metastasis,muscle invasion and FIGO stage(P=0.028,0.031;P=0.004,0.016;P=0.008,0.011).Spearman rank correlation analysis showed there was positive correlation between ZNF139 and MMP-7 in adenocarcinoma tissues(r=0.716,P=0.039).Western blot showed that ZNF139 and MMP-7 expression in adenocarcinoma group were higher than in adjacent cancer group(t=6.92,7.34;P<0.01);qRT-PCR showed that ZNF139 mRNA and MMP-7 mRNA expression in adenocarcinoma group were higher than in adjacent cancer group(t=4.27,4.06;P<0.05).Conclusion ZNF139 and MMP-7 are correlated with malignant pathological characteristics in endometrial adenocarcinoma,they may modulate the malignant behavior together.
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Objective To observe the effect of everolimus on proliferation,cycle and apoptosis of endometrial cancer cells and discuss the mechanism.Methods Proliferation of RL95-2 interfered with everolimus in different concentration and time was observed by MTT assay.Cell cycle and apoptosis affected by everolimus was detected by flow cytometry.PI3K-Akt-mTOR signaling pathway was detected by Western blot.Results (1) MTT assay showed that,cell viability in everolimus group was time-dependent decreased,compared with control group,there were significant differences in 48 h,72 h and 96 h ((64.36±4.78)％,(48.18±5.93) ％,(42.72±6.91) ％;F inner grouP =3.278,P＜ 0.05;F between groups =12.327,P＜ 0.01;F across groups=7.729,P＜0.05).Cell viability in everolimus group was dose-dependent decreased(F=3.264,P＜0.05).Compared with control group,RL95-2 cells in G1 phase and G2 phase was (69.28±2.61)％ and (4.75±0.84) ％,decreased in everolimus group,and in S phase (27.31±0.69) ％ was increased (t=5.743,P＜0.05;t =4.528,P＜0.05;t=6.209,P＜0.05).The apoptosis in everolimus group was 29.78％,higher than in control group (47.29％),the difference was significant (t =19.381,P＜0.01).(2) Western blot showed that both mTOR and p-Akt in everolimus group were dose-dependent decreased (F=3.589,P＜0.05;F =5.292,P＜0.05),Akt was dose-dependent increased (F =4.294,P＜0.05).Conclusion Everolimus decrease the cell viability and promote apoptosis via target inhibiting mTOR expression.
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OBJECTIVE:To investigate the characteristics and regularities of adverse drug reactions(ADR) induced by anti-infective drugs in our hospital.METHODS:657 ADR cases induced by anti-infective drugs collected by the ADR monitoring center in our hospital in 2006 were classified and analyzed.RESULTS:13 categories(49 kinds) of anti-infective drugs were involved in the total 657 ADR cases.Of which,those patients aged above 60 years accounted for 30.14%.The ADR were chiefly induced by quinolones,followed by cephalosporins and penicillins etc,which chiefly manifested as lesion of skin and its accessories(53.12%),followed by the lesions of digestive system and nervous system etc.CONCLUSION:Multiple factors contributed to the ADR,to which great importance should be attached so as to reduce the incidence of ADR induced by anti-infectives.
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Objective To study Axin expression in colorectal adenocarcinoma, colorectal adenoma, Peutz-Jeghers syndrome (PJS) polyp, and normal intestinal mucosa at mRNA and protein levels, and to explore the role of Axin in the pathogenesis of PJS. Methods The experiment samples, which were from the exsected normal mucosa, PJS polyps, adenomatous polyps and colorectal adenocarcinoma between Jan, 2001 and Mar, 2008 in Nanfang Hospital, each includes 32 sapamples. Immunohistochemistry was adopted to detect the Axin protein expression in each sample distinctively. Results transcriptase -polymerse chain reaction (RT-PCR) was used to detected and compare the Axin mRNA expression in 16 samples of each group. Results Of the tissues in each group, the positive Axin protein expression was located in the endochylema. The nomal mucosa group showed the most intensive expressive intensity, the olorectalc adenocarcinoma group was the least intensive, with significant difference in each group(P